DSpace Coleção:
https://repositorio.ufopa.edu.br/jspui/handle/123456789/5
2024-03-29T05:29:29ZAtividade antiedematogênica e antinociceptiva do óleo essencial de Pectis elongata Kunth (ASTERACEAE) quimiotipo citral
https://repositorio.ufopa.edu.br/jspui/handle/123456789/1575
Título: Atividade antiedematogênica e antinociceptiva do óleo essencial de Pectis elongata Kunth (ASTERACEAE) quimiotipo citral
Abstract: The species Pectis elongata Kunth, known in Pará as cumin or little lemon, is an easy-to-grow herbaceous plant, sold freely at fairs in Santarém and used by traditional communities as a spice and to treat pain and inflammation. Studies of the chemical composition of its essential oil revealed the presence of two distinct
chemotypes: limonene plus peryl aldehyde and citral (neral and geranial), whose anti- inflammatory and analgesic actions are already well established in the literature. The main hypothesis investigated in this research is that the essential oil obtained from P.elongata Kunth (EOPe) can serve as an alternative to standard therapy for pain and inflammation, minimizing adverse reactions such as gastrointestinal complications caused by the prolonged use of conventional drugs and that the presence of the chemotype, citral, with pronounced anti-inflammatory and analgesic action, in addition to having a gastroprotective effect, can help with adherence to treatment. For this, the objective of this work was to determine the EOPe chemotype of specimens collected in Santarém, Pará, and to evaluate its possible antiedematogenic (inflammation) and antinociceptive (pain) effect. The anti-inflammatory activity was induced by λ- carrageenan in a paw edema model in rats and the analgesic activity by formalin in mice. For this, a total of 36 rats and 36 mice were used, arranged in 6 groups, containing 6 animals each. For validation of tests, indomethacin and morphine were used as positive control (paw edema and formalin test, respectively), distilled water as the negative control, and mineral oil as solvent control. The oil yield after extraction was 1%. The chemical composition analysis showed the presence of the citral chemotype, representing 89.29% of the sample. In the antinociceptive activity test, EOPe showed analgesic activity only at a dose of 400 mg/kg, both in the neurogenic phase (78.22%) and in the inflammatory phase (58.03%), reducing pain behavior (licking and biting) of mice. The paw edema test in rats showed anti-inflammatory activity in reducing edema at doses of 200 mg/kg and 400 mg/kg, with maximum reduction values (70.2% and 70.5%, respectively) in the 1st hour of the experiment, followed by the 24th hour, with a reduction of (52.8% and 58.1%). Based on the results obtained, it is possible to suggest that EOPe, citral chemotype, has analgesic properties, inhibiting central and peripheral pain, in addition to promoting a reduction in paw edema, and showing anti-inflammatory potential.2022-01-01T00:00:00ZTratamento com corrente interferencial reduz a dor lombar e melhora a funcionalidade em pacientes com lombalgia crônica
https://repositorio.ufopa.edu.br/jspui/handle/123456789/1574
Título: Tratamento com corrente interferencial reduz a dor lombar e melhora a funcionalidade em pacientes com lombalgia crônica
Abstract: Low back pain is characterized by pain and represents a change in health that mainly affects adults, although electrostimulation represents an important tool in the treatment of patients with chronic nonspecific low back pain, the clinical efficacy of the interferential current used in physiotherapy still lacks reinforcement for clinical validation. Thus, in the present study, we evaluated whether the use of interferential current contributes to reducing chronic low back pain and analyzed whether the electrotherapeutic protocol can benefit the physical functionality of patients with chronic low back pain. 24 individuals were evaluated, with low back pain for more than 12 weeks and aged between 12 and 65 years. The current used in the lumbar region of the participants was of the tetrapolar interferential type and the stimulation mode was automatic vector, carrier frequency of 4,000 Hz, modulation frequency (AMF) of 50 Hz, SWEEP frequency of 20Hz. Pain and functionality assessment were performed using the data collection instrument the Visual Analog Pain Scale (VAS), Rolland Morris Disability Questionnaire (RMDQ) and the Owestry Disability Questionnaire (ODQ) respectively. Data analysis was performed using the JAMOVI 2.2.5 software. Our data demonstrate that individuals submitted to interferential current present significant improvement in pain relief, as well as in the functional pattern, opening new perspectives on the use of this therapeutic approach.2023-01-01T00:00:00ZÓleo essencial nano estruturado de Pectis elongata Kunth: potencial antimicrobiano, anti-inflamatório e estimulante do reparo tecidual de feridas cutâneas
https://repositorio.ufopa.edu.br/jspui/handle/123456789/1573
Título: Óleo essencial nano estruturado de Pectis elongata Kunth: potencial antimicrobiano, anti-inflamatório e estimulante do reparo tecidual de feridas cutâneas
Abstract: Pectis elongata Kunth, it’s an herbaceous plant, knowledge on the Northern Brazil like "cuminho-bravo", "alecrim limão" or "alecrim-bravo". It´s a species rich in essential oil, which present as majority constituent, the citral, component with recognized pharmacological properties. Nevertheless, it is known that essential oils present low solubility and high volatility, limiting its use as a therapeutic agent. In such a way, the insertion of essential oils in nanostructured systems, such as nanoemulsions, may be a promising alternative to circumvent the limitations related to its use. In the present study, our proposal was to evaluate the efficiency of the process of nanoemulsification of P. elongata essential oil about the retention of its main volatile constituents, as well as on its antimicrobial properties, anti-inflammatory and stimulating the healing process of cutaneous wounds. For this, in the first step, after obtaining the essential oil by hydrodistillation, the nanoemulsification process was performed using the low energy method and Tween80 as surfactant, resulting in particles with nanometric size (109.7 ± 1.4 nm), low polydispersity index (0.182 ± 0.00) and negative zeta potential (-32.3 ± 1.2 mV). These results characterize it as a nanoemulsion with uniform size distribution and repulsion forces strong enough to prevent coalescence between particles; properties that ensure its physical stability. To investigate the protection exercised by the nanostructured system against volatilization of the main components present in the essential oil of P. elongata, we determined the chemical composition of the free and nanoemulsified oil, using techniques of gas chromatography coupled to mass spectrometry. In this stage, the samples were stored under temperature of 25°C, during the period of 12 months, being the chemical analyses of the nanoemulsions performed at 0, 7-, 15-, 60- and 360-days post-preparation. The results showed citral as the major constituent for all samples analyzed where, in the free oil, the concentration of this compound was determined in 92.5%; and in the nanostructured system, in the post-preparation times quoted, the concentration of citral was determined in 92.8%, 92.6%, 90.8.0%, 95.7% and 78.9%, respectively. In the next step, we used the broth microdilution method to determine the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of the nanostructured essential oil, in the face of bacteria of interest in public health, which interfere in the process of cutaneous wound healing. The standard strains selected for study were Streptococcus pyogenes S012, Staphylococcus epidermidis S010 and Staphylococcus aureus S008. In this test, it was decided to evaluate the antimicrobial potential of nanoemulsions stored at 25°C, at the initial and final times of the storage period (0 and 360 days, respectively). It was verified that there was no significant loss of its antimicrobial potential after 360 days of storage. Finally, the anti-inflammatory activity was evaluated by the carrageenan-induced paw edema test, and the stimulating potential of the cicatricial process was evaluated on skin wounds induced by surgical incision. The anti-inflammatory activity of the nanoemulsion was observed only for the dose of 10 mg mL-1, presenting significant results when compared to the negative control (distilled water). In the healing results, no significant increase in the percentage of wound contraction was observed when compared to the negative control (sterile saline solution). From these results, it is possible to suggest that the process of nanostructuring the essential oil of P. Elongata, using the technique of low energy input, was able to produce a physically stable nanoemulsion, showing effectiveness both in maintaining its chemical properties and in preserve its biological properties, inhibiting the growth of microorganisms and modulating the inflammatory response induced by carrageenan. On the other hand, at the concentrations evaluated, the nanoemulsion was not able to accelerate the contraction process of skin wounds.2022-01-01T00:00:00ZExposição mercurial: perfil clínico-epidemiológico de mulheres na região do Baixo Tapajós
https://repositorio.ufopa.edu.br/jspui/handle/123456789/812
Título: Exposição mercurial: perfil clínico-epidemiológico de mulheres na região do Baixo Tapajós
Abstract: Mercury is a heavy metal that causes harm to human health. Mercury exposure occurs through means such as the burning of amalgam and the consumption of mercury-contaminated fish. Women of childbearing age are part of the vulnerable group and must be monitored; in order, above all, to avoid the harmful health effects of mercury exposure. Even more so when considering that the effects of mercury exposure affect the fetus in the intrauterine period, contributing to delays and/or damage to the nervous system, congenital malformations and
abortions. In the Brazilian Amazon, there is an interest in investigating mercury exposure, given the anthropic mining activities in the region, as well as the presence of contaminated fish that add risks to the health of the population, mainly due to the high consumption of fish by people from the Amazon region. Understanding that in the Baixo Tapajós region there is an environmentally exposed population and in view of the dangers that mercury exposure may have in women, this study aims to characterize the epidemiological and clinical profile of women environmentally exposed to mercury in the Baixo Tapajós region, Pará. This is a
descriptive, cross-sectional and quantitative study, which was carried out based on data collected from 2015 to 2019. The population sample of the present study is 327 women, living in urban, riverside and plateau areas of the region of Lower Tapajos. For the purposes of the research, social, demographic, food, anthropometric, clinical and laboratory variables were collected and analyzed. An analysis of the concentration of mercury in the blood was also performed, considering as “exposed” women with more than 10 μg/L of mercury. Descriptive
and inferential statistical analysis of these variables was performed using the STATA software (version 16). Based on the proposed data analysis, the results showed that 73.4% (n=240) of the women had mercury levels above 10 μg/L, of these 81.1% had basic schooling and were engaged in occupational activities related to fishing (93 .1%) and agriculture (88.6%). Among these exposed women, 70.5% (n=155) were of childbearing age, however, women over 50 years old were the participants with the highest mean blood mercury value of 41.4±51.3 μg/L and median of 21.2 μg/L. It was shown that 60.4% (n=145), that is, more than half of the women, lived in river areas and were exposed to high levels of mercury. Regarding the symptoms, those
related to the nervous and muscular systems were more frequent, it is noteworthy that the frequency of symptoms was three times higher among women exposed to high levels of mercury. The anthropometric evaluation showed a predominance of 56.9% (n=124) of overweight/obese women, of which 79% (n=98) had mercury levels above 10 μg/L, with an average value of 46 .6±59.6 μg/L. Regarding blood pressure, 49.3% (n=100) of the women had blood pressure levels ≥140x90mmHg, of these 82% (n=82) had mercury values above 10 μg/L. It is noteworthy that the proportion of overweight/obese women was more than three times
higher among women with high mercury levels, as well as altered blood pressure, which was 4.5 times more frequent in the subgroup of exposed women. The results of the present research demonstrate a profile of high mercury exposure in women of childbearing age, still portraying the strong influence of factors linked to social vulnerability, geographic isolation and high consumption of fish for the exposure of women to mercury. The results also point to the presence of evidence of the negative effects of mercury exposure on women's health, which encourages the development of more studies aimed at measuring them. In view of this health
scenario, actions must be planned for the intervention and control of mercury exposure of the population in the Amazon, for which what must be considered is the complex relationship established between the population and the environment. The social and economic profile of this population follows the influence of the countryside, forest and water, so solutions that aim to curb the consumption of fish tend to be impractical, even more so because they disregard the social vulnerability of this population. In order to successfully control and monitor mercury, appropriate strategies must be devised for the specificities of the territory and the population.
To this end, popular participation and empowerment on issues involving mercury, health and the environment are fundamental to the success of this process, with access to information being the first step.2022-07-07T00:00:00Z