Análise da viabilidade do gene 28s para a delimitação molecular do gênero Macrobrachium
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Data
2019Autor
http://lattes.cnpq.br/7273080712434334
RIBEIRO, Antônio Vitor Campêlo
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Crustaceans are an important ecologically and economically important group. The order
of the Decapods stands out for its diversity, but mainly for its relevance in the market,
because within this group are the individuals most consumed globally. However, there is
still a lack of techniques capable of making complete analyzes, since the identification is
mainly done in a morphological way, making room for errors and erroneous
classifications. One of the possible alternatives to solve this problem, provide reliable
data and generate reliable information is to combine several morphological,
morphometric, environmental and molecular approaches. With this, molecular biology
has begun to explore the use of molecular markers as a possibility, but this approach also
faces its limitations, one of which is the presence of heteroplasmy and pseudogenes in the
nucleotide sequences. These factors can lead to an erroneous amplification of the bases
and consequently few insurance data. An alternative to cure this problem is to make use
of more complete regions, having conserved and varied portions, with that, the use of the
28S gene for molecular identification of this group was started. This work aimed to
analyze the viability of the 28S gene in the molecular delimitation of Macrobrachium
through phylogenetic analyzes, intra and interspecific distance calculations and, finally,
ABGD analysis. For this 321 Genbank sequences were obtained and sorted out and
subjected to the above analyzes. The results of the Tree and ABGD were positive, since
the grouping was satisfactory, bringing together all the species close to each other. The
data from the distances confirmed that the barcode gap was necessary to distinguish
species within the genus. Therefore, the efficiency of this marker to discriminate species,
since the analyzes were shown to be congruent with the gene tree and with the intra and
intraspecific distances, evidencing the presence of a barcode can be used as a
complementary tool as an alternative identification the species that are difficult to
separate using only morphological data.