Indução de calogênese em explantes foliares de mandioca em resposta à suplementação exógena de auxinas
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2019-02-15Autor
http://lattes.cnpq.br/1793486947646165
COSTA, Tanara Pletsch Dalla
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The induction of callogenesis in cassava depends significantly on its genotype. Thus, it is
necessary to study the specific varieties of cassava in order to improve the techniques involved
in the callogenesis process. This work aims to evaluate the effects of different types and
concentrations of auxins in combination with 6-benzylaminopurine (BAP) on the callogenesis
induction from leaf explants of the BRS Kiriris cassava variety. The experimental design was
completely randomized, in a 3 x 3 factorial scheme, in which three auxins (naphthaleneacetic
acid - NAA, indoleacetic acid - IAA and indolebutyric acid - IBA) were used in concentrations
of 0.25, 0.5 and 1.0 mg L-1, with five replicates. A control treatment was performed, without
the addition of phytoregulators. After a 60-day in vitro culture, the percentage of calli formed,
the morphology of callus, number of calli, expansion, fresh weight and callus dry mass were
evaluated. Excepting the control treatment, calogenesis was confirmed in all treatments, with a
variation of 73 to 100% of callus formation. The IBA addition in concentrations of 0.5 and
1.0 mg L-1 provided the highest number of callus 1.78 and 1.77 calli, respectively. The NAA
supplementation was determinant for the greatest expansion (12.09 mm) and fresh (515.00 mg)
and dry mass (344.00 mg) of the calli. Therefore, calogenesis in leaf explants of the BRI Kiriris
cassava variety can be induced by the use of NAA, IAA and IBA auxins in combination with
BAP.