Desempenho de diferentes meios de cultura na quantificação da micobiota contaminante de piracuí no período chuvoso

Abstract

Piracuí is an artisanal product based on dehydrated fish that is widely sold at retail in Santarém, but is often stored/displayed in inappropriate places, conducive to microbial contamination. Thus, quantifying this contamination, particularly by fungi, is fundamental in the hygienic-sanitary evaluation of this product. In this research, fungal contamination was quantified in samples of piracuí sold in the markets of Santarém, during the rainy season, pointing out whether regional climatic conditions affect this contamination and indicating which culture medium is most suitable for such quantification. The samples were obtained at two fairs in Santarém and the cultivation was carried out in four culture media: Potato Dextrose Agar, Rose Bengal Agar, Glycerol Agar 18% and Saboraud Agar, all supplemented with 0.01% chloramphenicol. The results showed that the piracuí marketed in the city of Santarém has fungal contamination rates above the recommended level. This contamination, smaller in Fair , was influenced by factors linked to the fairs, which were statistically different from each other. There is a difference in this contamination between different vendors in each rainfall period. There was also an influence of the culture media in the detection of this contamination, with the BDA and SDA media being the most efficient and therefore recommended for quantification of the contaminating mycobiota of piracuí.