Comparação entre glicerina purificada aditivada mais metanol com milho moído e milho reidratado: parâmetros sanguíneos e ruminais

Abstract

The objective of this experiment was to evaluate ruminal and blood parameters of cows that received intraruminal pulses of pure glycerin, glycerin plus 7.2% methanol, finely ground corn grain, rehydrated and ensiled corn grain. The research was carried out at the Rosalina farm in the municipality of Santarém-PA. A 4x4 Latin square design was used with four treatments in four periods of five days each. The treatments consisted of finely ground corn grain (M), rehydrated and ensiled corn grain (MR), pure glycerin (G) or glycerin plus 7.2% methanol (GM), infused into the rumen in pulse dose on day 5 of the period experimental in a proportion of 0.5% of the live weight of each animal. Blood samples were analyzed for glucose and plasma urea nitrogen (PUN). For glucose and NUP analysis, blood samples were collected in tubes containing 6 mg of potassium fluoride and 12 mg of EDTA, centrifuged at 1118xg for 10min and the plasma frozen at -20°C until laboratory analysis. The NUP and glucose content were measured by colorimetric-enzymatic methods. Glycerin contaminated with up to 7.2% methanol did not alter ruminal and blood parameters.