Resposta imune inata do Colossoma macropomum suplementado com probiótico multi-espécies na sepse indizida por Aeromonas hydrophila em sistemas de recirculação de água clara e de bioflocos
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2023-06-23Autor
http://lattes.cnpq.br/9827972889977181
SILVA, Graziella Vivine Gonçalves de Matos
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This study evaluated the innate immune response and resistance to aeromonosis in Colossoma
macropomum supplemented with multi-species probiotics (Bacillus subtilis, Lactobacillus
plantarum and Pediococcus acidilactici), administered via feed and water, reared in clear water
and biofloc recirculation systems. The experimental design had two culture systems and three
treatments, supplemented with multi-species probiotic (AC CTL – control; AC PA – probiotic
in water; AC PR – probiotic in feed; AC PRA – probiotic in feed and water.; BFT CTL –
control; BFT PA – probiotic in water; BFT PR – probiotic in feed; BFT PRA – probiotic in feed
and water). The LD50% (9.0 x 10^8 CFU/mL) of the pathogen was determined and the
induction of sepsis by Aeromonas hydrophila was performed. There was an increase in the
production of ROS in the AC PA and BFT PR treatments. There was no lytic activity in the AC
system treatments, however, in the biofloc system, BFT PR and BFT PRA were effective in
improving this index. The serum serum lysozyme concentration was efficient in the AC and
BFT systems, when the probiotic was administered in the feed. The agglutination of serum
antibody titers did not result in benefits to the immunity of tambaqui supplemented with the
multistrain probiotic in the clear water and biofloc recirculation systems. After sepsis induction,
100% of the fish died in the AC system and more than 50% of the fish died in the BFT system.
In the present study, supplementation with the multi-species probiotic did little to improve the
innate immune response of tambaquis.