Desenvolvimento de diagnósticos moleculares para os agentes etiológicos das podridões de raízes de mandioca na Região do Oeste do Pará
Date
2022-10-25Author
http://lattes.cnpq.br/0240294274069368
SANTOS, Fernanda Cristina dos
Metadata
Show full item recordAbstract
Root rots are diseases caused by a complex of fungi and oomycetes, which in cassava
have a continuous and significant impact on production and consequently cause
socioeconomic damage to producing countries. For disease control, the correct diagnosis
of the pathogen is essential. Thus, the objective of this work was to develop a molecular
tool to differentiate the causal agents of cassava root rots through the ITS-RFLP of the
main causal agents known in Brazil (, Neoscytalidium dimidiatum, Phytophthora melonis,
Phytopythium sp., complexes Fusarium solani and Lasiodiplodia theobromae) digested
by selected enzymes in silico (EcoRI, BmgBI, HaeIII, SmaI and XhoI). It was possible to
verify the differentiation of the phytopathogens in two groups (ascomycetes and
oomycetes), only by the polymorphism of the fragment size of the ITS region.
Furthermore, it was possible to differentiate dry rot from black rot (Neoscytalidium
dimidiatum, complexes Fusarium solani, e Lasiodiplodia theobromae) using the enzymes
HaeIII, SmaI and EcoRI, as well as, within the oomycetes, to differentiate the species (P.
melonis, Phytopythium sp.) through the enzymes BmgBI and XhoI. Therefore, the ITSRFLP can be a new molecular tool for the rapid and efficient diagnosis of the causal
agents of cassava root rot in the main producing regions.