Efeito de Plathymenia reticulata sobre a toxicidade induzida por metilmercúrio, em ratos
Fecha
2015-11Autor
http://lattes.cnpq.br/6306918978793226
ALMEIDA, Juliana Gama de
Metadatos
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Mercury (Hg) is a known metal high toxicity been described over the years.
Methylmercury (MeHg) is one of organic forms of this metal that has a higher toxicity
find it easier to penetrate the cell membranes of organs and blood-brain and placental
barriers causing numerous systemic consequences in the contaminated organism. One of
the mechanisms underlying the toxicity of MeHg is to generate in excess of reactive
oxygen species (ROS) causing oxidative stress. P.reticulata is a species commonly
known as mahogany whose use in folk medicine is widespread mainly in northern and
central-western Brazil. The P. reticulata bark extract has in its composition tannins,
flavonoids and other phenolic groups of compounds of structure responsible in large
part by the antioxidant effect of the plant. The aim of this study was to investigate the
effect of aqueous extract of P. reticulata by contamination MeHg for 21 days in mice
through behavioral tests and Hg dosages in liver, kidney, brain and cerebellum. In vitro
testing was also carried out to evaluate antioxidant activity of the aqueous extract
through the superoxide radical test. They were selected for the 64 mice experiments
Wistar weighing males between 150 and 200g from the Vivarium of Bioprospecting
Laboratory and Experimental Biology (LabbBex) of the Federal University of Western
Pará. The animals were separated into the following groups (G) with n = 6 in each: G1
Water, G2 - P.reticulata 60 mg / kg, G3 - P. reticulata 120mg / kg, G4 - P. reticulata
240mg / kg, G5 - MeHg 4 mg / kg, G6 - P. reticulata 60mg / kg + MeHg , G7 - P.
reticulata 120mg / kg + MeHg, G8 - P. reticulata 240 mg / kg + MeHg. After 21 days
of daily administration animals were subjected to behavioral testing (Y maze, Box
Light-dark and Rota-Rod) subsequently euthanized and finally collecting made brain,
liver and kidneys for analysis. The results of the behavioral tests showed that the groups
that had received MeHg short and long term memory plus motor function affected. In
the Y maze, the switching% of G1, G2, G3, G4, G5, G6, G7 and G8 were on average
65; 62; 71; 62; 33; 75; 41; 20% respectively. In the rotating rod test it was observed that
the animals G5, G6 spent less time on the swivel bar, averaging 65.75. G7 and G8
animals were not able to remain on the swivel bar. Hg dosages showed higher
concentration of metal respectively in liver, kidney, brain and cerebellum. The dose of
P. reticulata 60 mg / kg proved to be more effective overall in protecting the damage
caused by metal to organs and memory and learning assessed in behavioral tests. The in
vitro evaluation of the extract demonstrated antioxidant activity through inhibition of
superoxide radicals (its% inhibition: 25.6%, 50.92% 68% 93% for 5 mg / mL
concentrations, 10 mg / mL, 20 ug / ml and 50 ug / ml.