Sejam bem-vindos ao Poraquê, o Repositório Institucional (RI) da Universidade Federal do Oeste do Pará (Ufopa). Nosso objetivo é armazenar, preservar, dar acesso e visibilidade à produção científica em formato digital da universidade. O nome Poraquê é uma alusão ao peixe-elétrico da Amazônia, em que seu corpo é capaz de produzir pulsos elétricos, é dito, que enxergar no escuro, logo, possui autonomia energética, capaz de acender uma lâmpada

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    Atividade biológica e investigação de compostos obtidos de Streptomyces sp. isolado de sedimentos do Lago Verde em Alter do Chão
    (2025-04-30) VASCONCELOS, KAMILA BRIELLE PANTOJA; SILVA, Silvia Katrine Rabelo da; https://orcid.org/0000-0002-9408-131X; http://lattes.cnpq.br/6999618172919722; https://orcid.org/0000-0003-0490-523X; http://lattes.cnpq.br/5820010356210887; SILVA, Silvia Katrine Rabelo da; https://orcid.org/0000-0002-9408-131X; http://lattes.cnpq.br/6999618172919722; SANTOS, Thalis Ferreira dos; http://lattes.cnpq.br/4212001504561883; RAMOS, Sara Freitas de Sousa; xxxxx; xxxx; COSTA, Isabella Cristhina Gonçalves; https://orcid.org/0000-0002-2948-4892; http://lattes.cnpq.br/2296052960250505
    One of the major challenges in public health is the resistance of pathogens to various drugs, leading to intense searches for new antimicrobial agents from natural sources, through the exploration of new niches. The objective of this research was to investigate actinobacteria from sediments of Lago Verde in Alter do Chão, Pará, Brazil, evaluating their antibacterial, cytotoxic and antioxidant activity, as well as investigating the chemical profile of the volatile compounds present in the crude extract. Identification occurred through morphological and biochemical evaluation. Their antibacterial activity was investigated by the agar disk method (6 mm), against 17 pathogens of clinical interest. Cytotoxic activity was determined by the percentage inhibition of cell growth in samples of the human colorectal carcinoma cell line HCT116, using a concentration of 10 μg/mL, with the results expressed as mean ± SEM (n = 2). Antioxidant activity was determined by the DPPH radical neutralization assay. The chemical profile of the volatile compounds in the crude extract was established by gas chromatography-mass spectrometry (GC-MS) analysis. The identification of the isolate LV20 presents characteristics of the phylum Actinobacteria, compatible with the genus Streptomyces; its antibacterial activity demonstrated action against Staphylococcus epidermidis (17.75 ± 1.25) and Enterococcus faecalis (17.5 ± 0.5), in addition to reducing the HCT116 tumor cell line, with 95.55% inhibition. The crude extract of LV20 showed significant antioxidant activity by the DPPH method, evidenced by a 50% Effective Concentration (EC) of 890.29 μg/mL. The chromatographic analysis identified bioactive volatile compounds, with emphasis on glycerol (28%), tris(2,4-di-tert-butylphenyl)phosphate (22.12) and benzenepropanoic acid, 3,5-bis(1,1- dimethylethyl)-4-hydroxyoctadecyl (14.67%), which present pharmacological potential. The unprecedented results demonstrate the biological variability of Streptomyces sp. LV20 as a promising source of bioactive molecules with therapeutic applications, highlighting the importance of Amazonian biodiversity for the bioprospecting of new drugs as an alternative in the face of microbial resistance.
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    Exposição ao mercúrio em comunidades da Região do Baixo Tapajós
    (Universidade Federal do Oeste do Pará, 2025-03-12) SOUZA, Marina Vasconcelos; MENESES, Heloisa do Nascimento de Moura; https://orcid.org/0000-0003-1996-5956; http://lattes.cnpq.br/3672995916961731; http://lattes.cnpq.br/1253594282390258; MENESES, Heloisa do Nascimento de Moura; https://orcid.org/0000-0003-1996-5956; http://lattes.cnpq.br/3672995916961731; OLIVEIRA, Sheyla Maria Silva de; LACERDA, Eliza Maria da Costa Brito; xxxxxx; xxxxx; FERNANDES, Franciane de Paula Fernandes; https://orcid.org/0000-0002-4617-1919; http://lattes.cnpq.br/8840851253152352
    Mercury (Hg) is a highly toxic element for human health. It can be derived from sources in the Earth's crust and from anthropogenic sources. The biotransformation, biomagnification and bioaccumulation of this metal in the ecosystem affects humans. One of its chemical forms, methylmercury, has the ability to accumulate in aquatic plants and fish and, thus, promote chronic exposure to Hg in people. Thus, the objective of this study is to characterize exposure to Hg in communities in the riverside region of the lower Tapajós, state of Pará. This is a cross-sectional, descriptive and quantitative research. The data in this study refer to collections carried out in 2022, in the communities of the lower Tapajós region. People of both sexes, aged 18 and over, with fixed residence in the riverside area for more than one year were included in this research. To collect the data, a questionnaire with sociodemographic, dietary and health questions was applied. In addition, 5 mL of venous blood was collected from each volunteer to measure total mercury (HgT). HgT measurements were performed using a DMA-80 Direct Mercury Analyzer, which quantifies Hg levels using atomic absorption spectrometry. For this study, the safety limit for Hg of 10 μg/L established by the World Health Organization (WHO) was considered. Descriptive analysis, daily Hg intake estimation, risk ratio calculation, Hg comparison tests (Kruskal-Wallis and Mann-Whitney tests) and Spearman correlation were performed. Statistical analyses were performed using JASP software, version 0.19.2.0. R software was used for Mann-Whitney analyses and to prepare boxplot graphs using the ggplot2 package. In this study, a significance level of p < 0.05 was adopted. Of the 570 individuals, females were the most present (63.33%). HgT levels were higher among men (median = 26.28 μg/L). Daily fish consumption was reported by 36.84% of participants, with a median HgT of 27.49 μg/L. Hg exposure is directly related to the total amount of fish consumed, with a strong positive correlation (ρ = 0.947, p < 0.001). A weak and negative correlation was observed between Hg levels and fruit consumption (ρ = -0.104, p = 0.013). There was a health risk, especially for women of childbearing age and based on the consumption of carnivorous fish. The risk estimate associated with fish consumption revealed that women of childbearing age have a risk ratio (RR) of 5.4, while adults of both sexes have a RR of 2.6. A statistically significant difference was found in the symptom of numbness in the hands (p = 0.0173) with median = 23.63 μg/L and body tremor (p = 0.047) with median = 23.43 μg/L. The riverside dwellers presented Hg levels above the WHO safety limit and are at risk to their health. Dietary patterns influenced Hg exposure. Hg exposure in the Tapajós River basin continues to be a public health concern, especially for riverside populations, as they depend on fish as their main food source. Therefore, mitigation measures, such as diversifying the diet with species from different trophic levels, encouraging the consumption of foods rich in antioxidants, and implementing educational campaigns on the risks of Hg exposure, are essential to reduce the health impacts of these populations. In addition, there is an urgent need to implement public policies aimed at environmental and health monitoring of populations exposed to Hg in the Amazon. Finally, further studies need to be carried out to clarify the association between the presence of symptoms that may be caused by the toxic and neurotoxic effects of Hg and exposure levels, as well as research that may determine possible risk markers for vulnerable populations.
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    Absenteísmo por doença e acidente em serviço entre servidores públicos federais: um panorama em uma universidade no interior da Amazônia
    (Universidade Federal do Oeste do Pará, 2025-05-26) ACORDI, Marlei Cristina; MORAES, Waldiney Pires; http://lattes.cnpq.br/2787433606563598; MORAES, Waldiney Pires; https://orcid.org/0000-0001-8524-3009; http://lattes.cnpq.br/5222632994807027; NICARETTA, Bruno Alexandre da Silva; https://orcid.org/0000-0001-8801-0761; http://lattes.cnpq.br/5493200188192899; PEREIRA, Rayanne Rocha; https://orcid.org/0000-0002-1995-9241; http://lattes.cnpq.br/3964604515231067; FERNANDES, Franciane de Paula; https://orcid.org/0000-0002-4617-1919; http://lattes.cnpq.br/8840851253152352
    Temporary work absence is defined as absenteeism. When the absences occur due to health reasons, it is classified as sickness absenteeism. As a complex and multifactorial phenomenon, it can be analyzed from various perspectives, with health being a significant focus of concern in many countries, as it can indicate workers’ health and working conditions. Due to the scarcity of data on the health and occupational accidents of federal public servants, particularly in the Northern region of Brazil, this topic requires investigation. This study aims to identify the characteristics of sickness absenteeism and work-related accidents among federal public servants at the Federal University of Western Pará (Ufopa). It is an observational, cross- sectional, and descriptive study based on absences approved by the SIASS Tapajós medical board between January 2019 and December 2023. The research was approved by the Ethics Committee, and the absence indicators were analyzed considering the number of absence episodes, the number of absent workers, and lost workdays using descriptive statistics. Results for the period include: 10.53% prevalence; absence frequency index of 16.73%; absenteeism duration of 52.62 days; and average absence duration of 33.12 days. Administrative staff, women, and the 31-40 age group stood out. Mental and behavioral disorders, infectious and parasitic diseases, and musculoskeletal system and connective tissue diseases were prominent in the number of absences. Similarly, these disorders and neoplasms stood out in the number of lost workdays. Fifteen occupational accidents were recorded, with a prevalence of 0.17% and an average absence duration of 13.78 days, accounting for 0.41% of the total lost workdays. Injuries, intoxications, and other external causes predominated. The prominence of infectious and parasitic diseases in absenteeism indices suggests the influence of the Covid-19 pandemic. The analysis of occupational accidents, rarely studied for their impact on public service absenteeism, proved innovative. The identified panorama may support policies and actions for health surveillance, accident prevention, and health promotion among public servants. Additional studies are needed.
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    Percepções de gestantes sobre os riscos da exposição ao mercúrio: um estudo qualitativo na Amazônia Brasileira
    (Universidade Federal do Oeste do Pará, 2025-03-20) REGO, Raissa Vasconcelos; OLIVEIRA, Sheyla Mara Silva de; MENESES, Heloisa do Nascimento de Moura; https://orcid.org/0000-0003-1996-5956; http://lattes.cnpq.br/3672995916961731; https://orcid.org/0000-0003-1637-7809; http://lattes.cnpq.br/5470158511972382; MENESES, Heloisa do Nascimento de Moura; https://orcid.org/0000-0003-1996-5956; http://lattes.cnpq.br/3672995916961731; LIMA, Juliana Gagno; https://orcid.org/0000-0002-5576-0002; http://lattes.cnpq.br/5426198496044277; MESCHEDE, Marina Smidt Celere; https://orcid.org/0000-0002-6519-9466; http://lattes.cnpq.br/0315034444431020; FERNANDES, Franciane de Paula; https://orcid.org/0000-0002-4617-1919; http://lattes.cnpq.br/8840851253152352
    Methylmercury (CH3Hg) is the form most harmful to human health and is made available in the Amazon ecosystem mainly through deforestation, burning, soil silting, illegal mining activities, industrial activities, predominant logging activities, exacerbated land use, the implementation of hydroelectric power plants, intensifying the effects caused by intoxication and contamination of the environment. In view of this, vulnerable groups such as pregnant women, riverside communities and fishermen are at risk, especially pregnant women, due to the nephrotoxic effects and impaired cognitive development of the fetus. Furthermore, the effects of mercury exposure go beyond the clinical aspects to include nutritional, cultural and socio-environmental aspects, compromising the logic of good living and the right to health. Therefore, this study investigated the perceptions of pregnant women about mercury exposure, its sources and environmental impacts, exploring the relationship between their eating habits, especially fish consumption, and mercury contamination, as well as analyzing the guidance received during prenatal care in Basic Health Units (BHU), focusing on the risks of mercury to fetal health. This is an exploratory, cross-sectional, field study with a qualitative approach, which was carried out in the Lower Tapajós region, Santarém (PA), in the years 2023 and 2024. This study was approved by the Research Ethics Committee (CEP) of the Federal University of Western Pará (UFOPA) under CAAE: 68121023.7.0000.017. The study involved 16 pregnant women attending Basic Health Units in the municipality of Santarém-Pa. Four thematic categories emerged from the interviews: : I- Mercury: grasping concepts of an unknown reality; II- Exposure and impacts on maternal health, revealing concerns about fetal malformations; III- Environmental changes, relating alterations in the territory to the impact of exposure: my territory is my history; IV- Food Safety: Transversalities of mercurial exposure. The study revealed that there was little dissemination of information during prenatal care on the subject of mercury to pregnant women, highlighting the need for continuous educational actions integrated into the local and regional context. The research also revealed that pregnant women were unaware of the risks of mercury poisoning and its implications for maternal and fetal health, highlighting the need to include guidance on safe fish consumption in prenatal consultations. It also highlighted the relationship between environmental changes and food security in riverside communities, suggesting the integration of educational and nutritional actions into publicpolicies. In the meantime, there is an urgent need for longitudinal studies and integrated health, education and environmental policies in order to mitigate risks in vulnerable populations.
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    Estratégias lúdicas para o ensino de língua inglesa: estudo de caso único
    (Universidade Federal do Oeste do Pará, 2019) OLIVEIRA, Isabela de Souza de; HALL, Silvia Cristina Barros de Souza; https://orcid.org/0000-0002-4299-2867; http://lattes.cnpq.br/1900825503546910; http://lattes.cnpq.br/4559945631539033; Paola Piovezan Ferro; https://orcid.org/0000-0002-5805-9422; http://lattes.cnpq.br/0999668454079390; Maria Luiza Fernandes da Silva Pimentel; http://lattes.cnpq.br/3596876678287554
    This work aimed to explore playful strategies in order to help the process of teaching and learning the English language for children. For this matter, I used the resources of the method known as action research where, with the participant’s help, I planned classes and materials to be used during the teaching process. The research was conducted through a single case study, involving a 16 six-year-old child, which was not having the first contact with the English language. The purpose of the analysis of the results obtained during this research was to understand if there would be effectiveness in the use of activities different from the traditional context, that is, how to teach in a completely different context of the classrooms, using different methodologies and materials, which could make studying more attractive and pleasurable, when it comes to teaching English language, besides observing the evolution and advancement in the area of subject knowledge. Thus, I identified the effectiveness of the type of methodology I used, observing the learning process through which the child went from the first contact to the last, and finally, sought to report the progress of the improvement of speech and writing of the child in relation to the English language.