Atividade antimicrobiana do mel de abelha sem ferrão associado à nanopartículas de prata

Abstract

Since antiquity, honey has been used empirically to treat diseases and wounds, but only in the last decades have they started to develop studies to analyze its anti-inflammatory, antimicrobial, healing and tissue stimulating properties. Considering these properties, the objective of this research is to investigate the antimicrobial activity of honey in natura and, then, to make the solution of the same honey added to the silver nanoparticles, known for having disinfectant and antiseptic properties, for the observation of a supposed enhancement antimicrobial effect of honey, and in both phases against the same microorganisms of clinical interest: Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Candida kruzei, using a well diffusion method. This is an analytical, experimental and bibliographic study. The sample will consist of 17 honeys from Apis mellifera and Melipona sp. produced in communities in Santarém-PA, Brazil. The physical-chemical and biochemical analyzes carried out on the honeys were: antioxidant activity (% of DPPH Inhibition),total phenolic compounds (mgGA100g- 1), reducing sugar content (%), pH, soluble solids content (° Brix), moisture (%). The use of AgNP is growing exponentially and with multiple applications and benefits, in addition to being able to suppress microbial growth in contaminated areas. Thus, in this study the effects of the association of honey with the silver nanoparticle were synergistic. The prepared biogenic AgNPs showed a cytotoxic effect against some Gram positive and negative bacteria. The green synthesis of AgNPs using different honeys can be an efficient and economical alternative to combat this bacterial multidrug resistance.