Resposta imune inata do Colossoma macropomum suplementado com probiótico multi-espécies na sepse indizida por Aeromonas hydrophila em sistemas de recirculação de água clara e de bioflocos

Abstract

This study evaluated the innate immune response and resistance to aeromonosis in Colossoma macropomum supplemented with multi-species probiotics (Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici), administered via feed and water, reared in clear water and biofloc recirculation systems. The experimental design had two culture systems and three treatments, supplemented with multi-species probiotic (AC CTL – control; AC PA – probiotic in water; AC PR – probiotic in feed; AC PRA – probiotic in feed and water.; BFT CTL – control; BFT PA – probiotic in water; BFT PR – probiotic in feed; BFT PRA – probiotic in feed and water). The LD50% (9.0 x 10^8 CFU/mL) of the pathogen was determined and the induction of sepsis by Aeromonas hydrophila was performed. There was an increase in the production of ROS in the AC PA and BFT PR treatments. There was no lytic activity in the AC system treatments, however, in the biofloc system, BFT PR and BFT PRA were effective in improving this index. The serum serum lysozyme concentration was efficient in the AC and BFT systems, when the probiotic was administered in the feed. The agglutination of serum antibody titers did not result in benefits to the immunity of tambaqui supplemented with the multistrain probiotic in the clear water and biofloc recirculation systems. After sepsis induction, 100% of the fish died in the AC system and more than 50% of the fish died in the BFT system. In the present study, supplementation with the multi-species probiotic did little to improve the innate immune response of tambaquis.