Actinobactérias nativas da rizosfera de Aniba parviflora Syn Fragans (macacaporanga) produtoras de substâncias biossurfactantes

Abstract

There is a growing demand for natural sources of products for the most diverse uses, such as industries and bioremediation methods, since bioproducts have advantages such as less toxicity and waste generation. Among the bioproducing microorganisms are actinobacteria, recognized for producing a variety of bioactive molecules, such as antibiotics, antitumorals, enzymes and biosurfactants. In this study, the ability of strains of actinobacteria isolated from the rhizosphere of Aniba parviflora Syn Fragans to produce biosurfactant substances was evaluated, evaluating their emulsifying action on lipid and fossil substrates. The bacterial strains were isolated and identified through morphological, biochemical and molecular data. Subsequently, the selection of the strains producing the lipase, esterase and hemolysin enzymes was carried out, and the strains with the highest enzymatic indexes were selected for the tests of biosurfactant production in liquid medium. The 11 strains isolated were identified as belonging to the genus Streptomyces sp., And the values of emulsification index found are promising when compared to studies with the genus Bacillus. The highest emulsifying action was recorded for the bacterial culture liquid of Streptomyces sp. MPO2 and Streptomyces sp. MPO11, indicating that the biosurfactant action is the result of molecules associated with bacterial biomass.