Estudo da aplicabilidade do DNA ribossomal 28S na identificação molecular de palaemonídeos da Amazônia Brasileira
Date
2020-04-30Author
http://lattes.cnpq.br/8487149226709579
ALVES, Danna Moraes
Metadata
Show full item recordAbstract
Carida shrimp make up a very diverse group within decapod crustaceans. Currently, 39 families
are recognized, with emphasis on the family Palaemonidae. In South America, palemonid
shrimp are the most specious family with wide distribution, and in the Brazilian Amazon the
presence of 33 species is registered, with emphasis on the genera Macrobrachium, Palaemon
and Pseudopalaemon. The literature for morphological and taxonomic analysis of palaemonids
is updated and well structured, however, this identification methodology is, in certain cases,
complex due to its great interspecific morphological homogeneity associated with considerable
intraspecific morphological variation. In the present work we aim to evaluate the applicability
of a 28S ribosomal DNA (rDNA) region as a tool for molecular identification of shrimp in the
Palaemonidae family. The composition of the database was through the collection of shrimp in
the vicinity of the municipality of Santarém, in the west of the State of Pará, where the
confluence of the Amazon River and the Tapajós River is located, and the incorporation of
samples specimens deposited in three museums in the northern region. The sequences were
organized as follows: (1) a database with 47 museum sequences and a phylogenetic tree was
built using Neighbor Joining method (NJ), based on simple genetic distance (p); (2) database
with 15 museum sequences and 27 streams of haplotype samples from streams and three species
delimitation methods were applied: ABGD, GMYC and PTP. With the clusters strongly
supported, the methodology used allowed an analysis by means of the correspondence between
the positioning of the sequences in different delimitation methods, where 20 species were
identified by the ABGD and GMYC approach and 30 species by the PTP method, we infer the
taxonomic identification of 16 haplotypes and a museum sample, we corrected the identification
of six museum samples, and 11 haplotypes were identified only at the gender level. The results
of the molecular data confirm the applicability of the nuclear 28S gene as a molecular marker
for species identification.