Identificação, isolamento e síntese de bactéria nitrificante para compor protótipo de produto biotecnológico comercial: Biophyago redux – bioativação e saúde em filtros biológicos e bioflocos

Abstract

Live bacteria have become a protagonist as an active product in the development of biotechnological products, such as nitrifying bacteria, which are essential microorganisms in the nitrogen cycle, responsible for converting ammonia into nitrite and, subsequently, into nitrate. This process is called nitrification and is essential for the maintenance and quality of water and soil. In aquaculture, these microorganisms are a main part of the Biofloc culture system (BFT). This system stands out within aquaculture for using little or almost no water exchange due to microbial activity. Thus, BFT is capable of cultivating tons of fish ensuring both food security and environmental sustainability. In this sense, the main objective of this work was to identify, isolate and synthesize bacteria involved in the nitrogen cycle to compose a commercial biotechnological product called: Biophyago redux – bioactivation and health in biological filters and bioflocs. To this end, water samples enriched with pre-established bioflocs were collected in Tambaquis production tanks, bioreactor samples (activated sludge and biofilm). Next, the lineage of nitrifying bacteria was isolated and identified from the promising strains. After identification and isolation, biochemical tests were carried out following Bergey's manual and in vitro tests in a controlled environment with the purpose of evaluating the performance and viability of the selected strains. A quantity of 5 strains was then obtained for each sample, making a total of 20 strains, of which 5 showed promising macro and microscopic nitrifying characteristics. Which then went on to biochemical tests. Of this quantity, there was no success in the T1 sample, while 01 strain from the T2 sample presented desirable characteristics, as for the BRLA sample, 02 strains were promising, from the BRBF sample, 02 strains demonstrated satisfactory results and went on to in vitro tests. In in vitro tests, at the end of the experiments, 03 promising nitrifying bacteria were obtained, which should make up the Biophyago redux product. Finally, the project seeks to collaborate with fish farming and society by making innovative biotechnological products available to the market that will result in savings in fish farming, improving the quality of the water used and the quality of the fish. Accordingly, it is expected to balance the need to innovate in the aquaculture process with the need to strengthen producers' profitability and environmental sustainability.